Quantitative Determination of Isotopic Abundance Ratio of 13C, 2H, and 18O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers

O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic intermediates, mostly used in medicines and pesticides. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, (PM+1)/PM and 18O/16O, (PM+2)/PM, in toluic acid isomers using gas chromatography-mass spectrometry (GC-MS). The OTA and MTA samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to biofield energy treatment. The GC-MS spectra of both the isomers showed five m/z peaks due to the molecular ion peak and fragmented peaks of toluic acid derivatives. The isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM were calculated for both the isomers and found significant alteration in the treated isomers. The isotopic abundance ratio of (PM+1)/PM in treated samples of OTA was decreased and then slightly increased upto 2.37% in T2, where the (PM+2)/PM in treated OTA, significantly decreased by 55.3% in T3 sample. Similarly, in case of MTA, the isotopic abundance ratio of (PM+1)/PM in the treated sample showed a slight increase the (PM+2)/PM was decreased by 11.95% in T2 as compared to their respective control. GC-MS data suggests that the biofield energy treatment on toluic acid isomers had significantly altered the isotopic abundance of 2H, 13C, and 18O in OTA and MTA as compared to the control.

In summary, toluic acid isomers were studied with GC-MS under the influence of biofield energy treatment and observed a significant change in isotope abundance of 2H/1H, 13C/12C and 18O/16O as compared to the control sample. The alteration in isotopic abundance ratios have significant impact on bond energies and chemical reactivity of the molecules. The smaller enhancement in the isotopic abundance ratio of (PM+1)/PM in OTA and MTA, the reactivity may be reduced slightly at the aromatic ring by slight increase in the effective mass (µ) and the binding energy of the treated sample. However, significant depletion in heavier isotopic abundance ratio, (PM+2)/PM, may increase the reactivity of the –COOH group after biofield energy treatment. From the above observations, it can be concluded that the enhancement of heavier isotopes in the aromatic ring may decrease the reactivity of the aromatic ring but decreased heavier isotopes may increase the reactivity of the functional group of toluic acid isomers, consequently enabling them to be used as an intermediate in the organic synthesis. It will also provide quicker biodegradation pathway in the environment while leaching.