Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone

Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated BS and PP samples were characterized by particle size analyzer (PSA), Brunauer-Emmett-Teller (BET) analysis, differential scanning calorimetry (DSC), x-ray diffraction (XRD), and thermogravimetric analysis (TGA). PSA results showed increase in particle size (d50 and d99) of both treated BS and PP as compared to control. Surface area analysis showed minimal decrease by 1.59%, in surface area of treated BS as compared to control. However, the treated PP showed increase (8%) in surface area as compared to control. DSC characterization showed increase in melting temperature of treated BS as compared to control. Whereas, DSC thermogram of treated PP showed decrease in melting temperature with respect to control. Moreover, the DSC of control and treated PP showed presence of exothermic peaks which were possibly due to protein aggregation. The treated PP showed higher exothermic transition temperature as compared to control. XRD analysis revealed slight reduction in crystalline nature of BS as compared to control. On the other hand, XRD data of control and treated PP showed an amorphous nature. TGA analysis of treated BS showed maximum thermal decomposition temperature at 22°C which was higher as compared to control sample (106°C). This could be due to biofield treatment which may enhance the thermal stability of treated BS with respect to control. However, the TGA thermogram of treated PP showed decrease in maximum thermal stability as compared to control. The overall results showed that biofield treatment has significantly altered the physical and thermal properties of BS and PP.

This research study has evaluated the influence of biofield treatment on thermal and physical properties of BS and PP. The treated BS showed increase in particle size (d50 and d99) as compared to control which might be due to fracturing of internal boundaries in the particles caused by biofield treatment. The treated PP also showed increase in particle size with respect to control; possibly due to biofield treatment and protein aggregation. Additionally, biofield treatment showed significant alteration in thermal nature of the treated samples. Based on the results the biofield treated BS and PP could be used in drug delivery systems. Further, Circular dichroism spectroscopic and Scanning electron microscopy/Transmission electron microscopy studies could be carried out to get further depth insights about thermal stability and aggregation nature of these treated organic products.