Evaluation of Biochemical Marker – Glutathione and DNA Fingerprinting of Biofield Energy Treated Oryza sativa

Food production needs to increase to satisfy the demand due to increasing human population worldwide. To minimize this food crisis, an increase in the rice production is necessary in many countries. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on rice (Oryza sativa) for its growth-germination of seedling, glutathione (GSH) content in seedling and mature plants, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of O. sativa cv, 644 was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi for biofield energy treatment and denoted as treated sample. The growth-germination of O. sativa seedling data exhibited that the biofield treated seeds was germinated faster on day 3 as compared to control (on day 5). The shoot and root length of seedling was slightly increased in the treated seeds of 10 days old with respect to untreated seeds. Moreover, the plant antioxidant i.e. GSH content in seedling and in mature plants was significantly increased by 639.26% and 56.24%, respectively as compared to untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was significantly (p<0.05) increased by 106.90% and 20.35%, respectively with respect to control. Besides, the DNA fingerprinting data using RAPD, revealed that the treated sample showed an average range of 5 to 46% of DNA polymorphism as compared to control. The overall results envisaged that the biofield energy treatment on rice seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the treatment of biofield energy on rice seeds could be used as an alternative way to increase the production of rice.

Based on study outcome, the biofield energy treated O. sativa showed faster with 100% germination as compared to the control. Moreover, the GSH content in treated sample was increased significantly by 639.26% of O. sativa seedling and 56.24% in mature plants as compared with their respective control. Apart from this, the plants growth regulating constituent IAA was also increased significantly by 106.90% in rice roots, while 20.35% was increased in rice shoots as compared to the control. RAPD analysis data of the treated sample showed an average range of 5 to 46% of polymorphism among the primers as compared to the control. In conclusion, the present investigation demonstrates that Mr. Trivedi’s unique biofield treatment could be utilized as an alternate therapeutic approach concurrent with other existing therapy to improve the productivity of rice in the field of agriculture in the near future.