Antibiogram of Biofield-Treated Shigella boydii: Global Burden of Infections

Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydiii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9207) strain of S. boydiii was used in this experiment. The study was conducted in revived and lyophilized state of S. boydiii. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. boydiii were subjected to Mr. Trivedi’s biofield treatment. Gr. II was assessed on day 5 and day 10, while Gr. III on day 10 with respect to control (Gr. I). Sensitivity pattern of amoxicillin/k-clavulanate was improved from intermediate (I) to susceptible (S) with correspond to MIC value was also reduced by two folds (16/8 to ≤ 8/4 μg/mL) in both the treated groups as compared to control. The antimicrobial susceptibility of S. boydiii showed 15% alteration in Gr. II on day 5, while significant (40%) alteration was found on day 10 as compared to control. The MIC values of antimicrobials for S. boydiii also showed 12.50% alteration in Gr. II on day 5 while, significant alteration (59.38%) of minimum inhibitory concentration (MIC) value was found in Gr. II on day 10 as compared to control. It was observed that overall 69.70% biochemical reactions were changed in which 66.67% alteration was found in Gr. II on day 10 with respect to control. Moreover, biotype numbers were changed in all the treated groups without alteration of organism as compared to control. These results suggested that biofield treatment had significant impact on S. boydiii in Gr. II on day 10 with respect to antimicrobial susceptibility, MIC and biochemical reactions pattern.

Altogether, the biofield treatment has significantly altered the susceptibility pattern (40%) with MIC values (59.38%) of tested antimicrobials against the ATCC strain of S. boydii in revived treated cells (Gr. II) as compared to control. It also altered significantly the biochemical reactions pattern (66.67%) of biofield treated strain of S. boydii in Gr. II as compared to control. On the basis of changed biochemical reactions of S. boydii the biotype numbers were altered in Gr. II and III without alteration of organism as compared to control. Mr. Trivedi’s biofield treatment could be applied as an alternative therapeutic approach to alter the sensitivity pattern of antimicrobials in near future including strict public health strategies like clean water supply, good sewage management and a clean environment against bacillary dysentery and acute gastroenteritis patients infected by S. boydii.