The Effect of Vitamin D3 on Bone Mineralization: Influence of Consciousness Energy Healing Treatment

The main objective of this study was to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Alan Joseph Balmer and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The test samples (BT) were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 317.97%, 245.94%, and 247.97% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10 µg/mL, while increased by 252.87% and 244.05% in the BT-DMEM + BT-Test item at 0.1 and 1 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 149.41% and 175.17% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 607.3%, 346.41%, and 197.61% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 31.98%, 54.87%, and 67.1% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 100 µg/mL compared to the untreated group. Additionally, the percent of bone mineralization was distinctly increased by 71.41%, 199.92%, and 32.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 28.29% and 25.59% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item group at 50 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, low bone density, bone, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

The MTT cell viability assay data showed more than 73% cells were viable, which indicated that the test samples were safe and nontoxic in all the tested concentrations. ALP was significantly increased by 317.97%, 245.94%, and 247.97% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10 µg/mL, while increased by 252.87% and 244.05% in the BT-DMEM + BT-Test item at 0.1 and 1 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 607.3%, 346.41%, and 197.61% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item group at 0.1 µg/mL, respectively compared to the untreated group. Additionally, at 100 µg/mL the level of collagen was also significantly increased by 31.98%, 54.87%, and 67.1% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the UT-DMEM + UT-Test item group. Further, the percent of bone mineralization was distinctly increased by 71.41%, 199.92%, and 32.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated test samples (The Trivedi Effect®) demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable for the development of an alternative and more effective supplement for vitamin D3 deficiency, which could be useful for the management of various bone related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, etc. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, inflammatory diseases, anti-inflammatory, anti-stress, anti-arthritic, anti-osteoporosis, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic actions stress management and prevention, and anti-aging by improving overall health, Parkinson’s Disease and stress etc. to modulate the immune system by improving overall health.