Role of Vitamin D3 on Bone Health in Human Bone Osteosarcoma Cells (MG-63): Influence of Biofield Energy Healing Treatment

The aim was to evaluate the impact of Biofield Treated vitamin D3 and DMEM on bone health. The test items (TI), were divided into two parts. One part of each sample received Consciousness Energy Treatment by Gopal Nayak and those samples were labeled as Biofield Treated (BT), while other parts of each sample were denoted as untreated test items (UT). MTT data showed test samples were found safe and nontoxic in tested concentrations. ALP was significantly increased by 213.46% and 218.66% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 1 µg/mL compared to UT-DMEM + UT-TI. Moreover, ALP was significantly increased by 200.79% and 187.09% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 10 µg/mL than untreated. Collagen synthesis was significantly increased by 551.25% and 130.31% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 1 µg/mL than untreated. Collagen was significantly increased by 401.97% in BT-DMEM + UT-TI at 10 µg/ mL, while increased by 197.57% and 276.82% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 50 µg/mL than untreated. Percent bone mineralization was significantly increased by 186.86% and 188.23% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 10 µg/mL than untreated. It was further increased significantly by 224.04% and 156.72% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 50 µg/ mL than untreated. Overall, data suggests that Biofield Treated vitamin D3 could be a potential alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone disorders.

The MTT cell viability assay data showed more than 74% cells were viable, which indicated that the test samples were safe and nontoxic in all the tested concentrations. ALP was significantly increased by 100.06%, 213.46%, and 218.66% in UT-DMEM + BTTest item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 1 µg/mL compared to the UT-DMEM + UT-Test item group. ALP was significantly increased by 95.98%, 200.79%, and 187.09% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 147.75%, 90.25%, and 105.29% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. The collagen synthesis was significantly increased by 551.25% and 130.31% in the BT-DMEM + UT-Test item and BTDMEM + BT-Test item groups, respectively at 1 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 401.97% and 89.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 10 µg/ mL compared to the untreated group. Further, the collagen level was significantly increased by 197.57% and 276.82% in UTDMEM + BT-Test item and BT-DMEM + UT-Test item, respectively at 50 µg/mL compared to untreated. Further, the percent of bone mineralization was distinctly increased by 186.86% and 188.23% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 10 µg/mL compared to the untreated group. The percentage of bone mineralization notably increased by 224.04% and 156.72% in the BT-DMEM + UT-Test item and BT-DMEM + BTTest item, respectively at 50 µg/mL with respect to the untreated group. Altogether, the Biofield Energy Treated test samples (The Trivedi Effect®) demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable for the development of an alternative and more effective supplement for vitamin D3 deficiency, which could be useful for the management of various bone related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, etc. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Addison Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Psoriasis, Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Irritable Bowel Syndrome, anti-inflammatory, anti-stress, anti-arthritic, anti-osteoporosis, anti-apoptotic, wound healing, anti-cancer, Besides, it can also be utilized in stress management and prevention, and anti-aging by improving overall health.