In Vitro Evaluation of Anti-Rheumatoid Arthritis Potential of Biofield Energy Treatment by Inhibition of IL-8 Secretion Using Synovial Sarcoma Cell Line (SW982)

Interleukin-8 (IL-8) has proven a significant role in the inflammatory rheumatic disorder, which is produced in the synovial fluid. The objective of this study was to investigate the effect of Consciousness Energy Healing based DMEM medium in SW982 (Synovial cell line) on bone health inflammatory parameter like interleukin-8 (IL-8). The test sample (DMEM medium) was divided into three parts, first part received one-time Biofield Treatment by a renowned Consciousness Energy Healer, Alice Branton and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times the Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. Cell viability using MTT assay showed that the cell viability of SW982 cells was more than 95% with a safe and non-toxic profile. Besides, the two-times Biofield Treated DMEM group (BT-II) showed a significant (p≤0.001) inhibition of IL-8 by 31.82% as compared to the untreated DMEM group under stimulation with IL-1β in SW982 cells. Thus, data reflected the significant potential of the test items in bone-health, which play a vital role in the promotion and maintenance of strong and healthy bones against rheumatoid arthritis. Overall, Consciousness Energy Healing Treatment demonstrated a significant inhibition of pro-inflammatory cytokine (IL-8) that signifies it’s potential to act as an anti-rheumatic action in arthritis and other bone inflammatory disorders along with autoimmune disorders such as osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, osteoma, aging, bone loss, and fractures.

MTT assay for cell viability showed significant improved cell viability with more than 95.8% among all the tested groups, which suggested that the test items were safe and nontoxic. In addition to, the effect of the two-times Biofield Energy Treated DMEM (BT-II) on IL-1β stimulated secretion of IL-8 in SW982 cells after 24 hours demonstrated a significant inhibition of IL-8 level by 31.82% as compared to the untreated DMEM group. Thus, the Biofield Energy Treated (The Trivedi Effect®) DMEM was found to have significant impact on rheumatoid arthritis, which is very vital to combat against various bone-related disorders. Thus, with respect to the untreated DMEM, Biofield Energy Treated DMEM would be highly significant in the growth of SW982 cells on bone health. Therefore, the Consciousness Energy Healing based DMEM might be a suitable alternative media for cell growth. It can be useful for the management of rheumatoid arthritis and against various bone-related disorders such asosteoma, osteoporosis, Paget’s disease of bone, osteomalacia, rickets, deformed bones, etc. Apart from it could be useful for the management of hormonal balance, stress, aging, and other bone disorders that occurs due to poor nutrition, genetically predefined, etc. Besides, it might be useful various immune-related disease conditions such as Sjogren Syndrome, Ulcerative Colitis, Dermatomyositis, Alzheimer’s Disease, Myasthenia Gravis, Dermatitis, Irritable Bowel Syndrome, Graves’ Disease, Asthma, Hashimoto Thyroiditis, Pernicious Anemia, Multiple Sclerosis,Systemic Lupus Erythematosus, Aplastic Anemia, Hepatitis, Atherosclerosis, Diverticulitis, Diabetes, Parkinson’s Disease, etc.