Improved Metabolism of Vitamin D3 in Human Osteoblasts Cells After Biofield Energy Healing Treatment

Bone formation, remodeling, mineralization, resorption, and healing processes involves a coordinated action of different cell types, which are very prevalent in the elderly population. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were studied. The cell viability using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 304.6%, 237.2%, and 110.6% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI, while 356.9%, 186.2%, and 87.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+BT-TI group as compared to the untreated group. Collagen content was significantly increased up to 163.7%, 114.4%, and 22.7% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 190.1%, 64.9%, and 50% increased collagen at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 330% and 121.5% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+BT-TI group, while 146.7% and 305.9% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+UT-TI group, as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium, and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Biofield Energy Healing based vitamin D3 and DMEM showed significant effects on bone health parameters in MG-63 cell line. The test samples were first screened for cell viability using MTT assay, which suggest a significant improved cell viability with more than 70% in all the test samples at various concentrations. Further, bone health parameters such as ALP data showed that the Biofield Energy Treated test samples showed a significant improved level of ALP by 144.6% and 127.7% in the UT-DMEM+BT-TI at 1 and 10 µg/mL, respectively, while 304.6%, 237.2%, and 110.6% increased at 1, 10, and 50 µg/mL in the BT-DMEM+UT-TI group. Additionally, the ALP level was increased by 356.9%, 186.2%, and 87.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. The level of collagen was significantly increased by 163.7% and 114.4% at 0.1 and 1 µg/mL, respectively in the UT-DMEM+BT-TI group, while 159.3%, 59.5%, and 40.2% increased collagen was reported at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, the level of collagen was increased by 190.1%, 64.9%, and 50% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated test group. Similarly, the bone mineralization percent was significantly increased by 44.3% at 10 µg/mL in the UT-DMEM+BT-TI group, while 146.7%, 305.9%, and 41.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI, and 330% and 121.5% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+BT-TI groups, respectively as compared with the untreated test group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, calcium and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoma, low bone density and osteoporosis, rickets, osteomalacia, bone and/or joint pain, osteogenesis imperfecta, Paget’s disease of bone, increased frequency of fractures, deformed bones, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-stress, anti-aging, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, cell cycling and proliferation, hormonal balance, neurotransmission, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Asthma, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Ulcerative Colitis, Alzheimer’s Disease, Aplastic Anemia, Hepatitis, Irritable Bowel Syndrome, Atherosclerosis, Sjogren Syndrome, Dermatitis, Diabetes, Diverticulitis, Dermatomyositis, Myasthenia Gravis, Pernicious Anemia, Systemic Lupus Erythematosus, Parkinson’s Disease, stress, etc. with a safe therapeutic index to improve overall health and quality of life.