Improved Metabolic Cardiac Biomarkers Activity Using Rat Cardiomyocytes Cell Line (H9c2) against Biofield Energy Treated Test Sample

Cardiac biomarkers are important targets for any test sample to discover ideal cardiac health supplement. The study was aimed to evaluate the cardioprotective activity of Biofield Energy Treated test sample (DMEM) using rat cardiomyocytes (H9c2) cells. The test item (DMEM medium) was divided into three parts, first part received one-time Biofield Treatment by Alice Branton, a renowned Biofield Energy Healer, and defined as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received two-times Biofield Energy Treatment and denoted as the BT-II DMEM. The third part did not give treatment and denoted as the untreated DMEM group. The cell viability of the test sample by 3-(4,5-Dimethylthiazol2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay showed an increase level of viable cells by 86.58% and 91.37% in the BT-I and BT-II groups, respectively. Besides, BT-I and BT-II groups showed 10.56% and 23.53% cytoprotective action compared to the tert-Butyl hydroperoxide (t-BHP) induced group. Further, lactate dehydrogenase (LDH) level was significantly (p≤0.001) reduced by 54.43% and 53.52% in the BT-I and BT-II groups, respectively compared to t-BHP induced group. Moreover, the level of creatine kinase-myocardial band (CK-MB) was reduced by 63.48% and 60.68% in the BT-I and BT-II groups, respectively compared to the t-BHP induced group. Additionally, reactive oxygen species (ROS) level was significantly inhibited by 55.6% and 112.8% in the BT-I and BT-II groups, respectively compared to the t-BHP induced group. Besides, apoptotic activity was significantly inhibited by 16% and 10% in the BT-I and BT-II groups, respectively compared to the t-BHP induced group. Overall, the experimental data demonstrated that The Trivedi Effect® has significant capacity to improve the overall cardiac parameters in H9c2 cells, which can be used against various cardiac disorders such as congestive heart failure (CHF), cardiomyopathy, heart valve disorders, arrhythmias, myocardial infarction, etc.

Cardiovascular action of the test samples after Biofield Energy Treatment suggested significant cardiac protection using various biomarkers in rat cardiomyocytes cell line (H9c2). The test samples were found to be safe and non-toxic in cell viability assay using MTT test with more than 86% viable cells. Besides, cellular protection data showed that one-time Biofield Energy Treated test item (BT-I) and two-times Biofield Energy Treated (BT-II) showed 10.56% and 23.53% cytoprotective activity, respectively as compared to the tBHP induced group. Further, the level of LDH was significantly suppressed by 54.43% in the BT-I group and 53.52% in the BT-II group as compared to the t-BHP induced group. The cardio-specific enzyme, creatine kinase-myocardial band (CK-MB) was significantly inhibited by 63.48% and 60.68% in the BT-I and BT-II groups, respectively compared to the t-BHP induced group. Moreover, the percent protection of reactive oxygen species (ROS) was found to be 55.6% and 112.8% in the BT-I and BT-II groups, respectively, compared to the t-BHP induced group. Apoptotic activity in H9c2 cells were significantly inhibited by 16% and 10% in the BT-I and BT-II groups, respectively compared to the t-BHP induced group. Overall, The Trivedi Effect® - Consciousness Energy Healing Treatment as a complementary and alternative treatment approach significantly improved all the vital cardiac biomarkers, which suggest its application in cardiac protection and cellular damage. Thus, it can be used for the prevention of various types of cardiac disorders such as stroke, heart attack, high blood pressure (hypertension), congenital heart disease, rheumatic heart disease, congestive heart failure (CHF), peripheral artery disease, valvular heart disease, carditis, thromboembolic disease, venous thrombosis, and many more. Moreover, the Consciousness Energy Healing Treatment could able to improve cell growth, differentiation, proliferation, neurotransmission, skin health, and communication from one cell to another cell. Besides, it can also be utilized against Pernicious Anemia, Parkinson’s disease, Dermatitis, Aplastic Anemia, Multiple Sclerosis, Myasthenia Gravis, Asthma, Irritable Bowel Syndrome, Hepatitis, Diabetes, Diverticulitis, Hashimoto Thyroiditis, Sjogren Syndrome, Atherosclerosis, and many more.