Implication of Biofield Energy Healing Based Vitamin D3 on Osteoblastic Differentiation

Bone is one of the dynamic organ of the endoskeleton, which play a vital role in maintain the structural integrity, blood production, coagulation, mineral reservoirs, and body immunity. The present study investigates the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in MG-63 cell line. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts, and each sample received the Consciousness Energy Healing Treatment by Su-Mei Chen Liu and labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone biomarkers were studied. The cell viability using MTT assay showed more than 84% in test items groups were found to be safe. ALP level was significantly increased by 217% (100 µg/mL), 272.8% (10 µg/mL), and 233.9% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 121.6%, 134.3%, and 86% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100 µg/mL as compared with the untreated group. Additionally, the percent of bone mineralization was significantly increased by 213.5% (at 10 µg/mL), 109.5% (at 100 µg/mL), and 283.7% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to improve the bone mineralization and calcium absorption. The bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Further, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss, and fractures.

Biofield Energy Healing based vitamin D3 and DMEM showed a significant improved bone health in various tested parameters in MG-63 cell line. MTT assay showed a significant improved cell viability with more than 84% in all the test samples at various tested concentrations. In addition, ALP data showed that the Biofield Energy Treated test samples in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups were reported with improved ALP by 217% (100 µg/mL), 272.8% (10 µg/mL), and 233.9% (100 µg/mL) respectively, as compared with the untreated group. Collagen level was significantly increased by 38.2% and 121.6% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI group, while 23.2% and 134.3% at 50 and 100 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, the level of collagen was increased by 70.4%, 73.7%, and 86% at 10, 50 and 100 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test group. Similarly, the bone mineralization percent was significantly increased by 213.5% and 11.5% at 10 and 100 µg/mL, respectively in the UT-DMEM+BT-TI group, while 47.2% and 109.5% at 10 and 100 µg/mL, respectively in the BT-DMEM+UT-TI groups. In addition, bone mineralization was increased by 283.7% at 100 µg/mL respectively in the BT-DMEM+BT-TI group as compared with the untreated test group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoma, rickets, low bone density and osteoporosis, osteomalacia, Paget’s disease of bone, bone and/or joint pain, osteogenesis imperfecta, increased frequency of fractures, deformed bones, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Ulcerative Colitis, Aplastic Anemia, Hepatitis, Diabetes, Asthma, Alzheimer’s Disease, Irritable Bowel Syndrome, Parkinson’s Disease, Graves’ Disease, Dermatitis, Dermatomyositis, Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Multiple Sclerosis, Myasthenia Gravis, Atherosclerosis, Diverticulitis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health and quality of life.