Impact of the Trivedi Effect®-Energy of Consciousness Healing on the Proliferation of Plant, Mouse and Human Stem Cells

The objective of the present study was to evaluate the effect of Biofield Energy Healing on the plant callus, mouse and human derived Mesenchymal Stem Cells (MSCs) for their cell count and cellular proliferation. All the three type stem cells and DMEM media for the stem cell culture were divided into two parts, one received Biofield Energy Treatment (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi through the Healer’s unique Energy Transmission process remotely and defined as Biofield Energy Treated (BT) group. On day 9 after treatment, the plant callus flasks of mandukparni, amla, and Centella were observed and visualized which showed an increased percentage in weight of the callus by 11.4%, 24.9%, and 51.7%, respectively as compared with the untreated groups. In addition, stem cell assay in murine bone marrow derived Mesenchymal Stem Cells (MSCs) showed an increased cellular proliferation by 115.2%, 127.6%, 161.9%, and 123.6%, in Biofield Energy Treated DMEM in the passage 1, 2, 3, and 4, respectively. Similarly, human derived Mesenchymal Stem Cells, AdiposeDerived (AD-MSC) cells showed a significant increased cell count by 139%, 119%, and 182% in the Biofield Energy Treated DMEM group with the passage 1, 2, and 3, respectively. Similarly, bone marrow (BM-MSC) cells showed a significant increased cell count by 102%, 170%, and 141% in the Biofield Energy Treated DMEM group with the passage 1, 2, and 3, respectively. However, BrdU assay for % cellular proliferation in the AD-MSC cells showed a significant increased cellular proliferation by 122%, 120%, and 184% in the passage 1, 2, and 3, respectively. On the other hand, BMMSC cells showed a significant increased cellular proliferation by 125%, 146%, and 157% in the passage 1, 2, and 3, respectively. Hence, the results suggest that there was a significant growth in plant callus weight, mouse and human stem cell growth and proliferation after The Trivedi Effect®-Energy of Consciousness Treatment. It can be concluded that The Trivedi Effect® can be used as a complementary and alternate therapy for the stem cells regeneration, improvement of tissue injury that might be every effective against various autoimmune human diseases such as arthritis, osteoarthritis, and different neurodegenerative disorders.

Biofield Energy Healing Treatment has been shown significant improvement of cell growth and proliferation of plant, mouse, and human stem cells. The Trivedi Effect®-Energy of Consciousness Healing Treatment on plant, mouse, and human stem cells showed significant growth (yield) of all the plant callus cultures as observed by phase contrast microscope. Overall, the plant yield (growth) of mandukparni, amla, and Centella callus increased by 11.4%, 24.9%, and 51.7%, respectively on day 9 as compared with the control. Similarly, the murine bone marrow derived Mesenchymal Stem Cells (MSCs) showed an increased cellular proliferation by 115.2%, 127.6%, 161.9%, and 123.6%, in BT-DMEM-P2, BTDMEM-P3, and BT-DMEM-P4, respectively. In addition, among human derived MSCs, AD-MSC cells showed a significant increased cell count by 139%, 119%, and 182% in the BT-DMEM-P1, BT-DMEM-P2, and BT-DMEM-P3, respectively as compared to the control group. Similarly, BM-MSC cells also showed a significant increased cell count by 102%, 170%, and 141% in the BT-DMEM-P1, BT-DMEM-P2, and BT-DMEM-P3, respectively. On other hand, BrdU assay in AD-MSC cells showed a significant increase % proliferation by 122%, 120%, and 184% in the BT-DMEM-P1, BT-DMEM-P2, and BT-DMEM-P3, respectively. BM-MSC cells showed a significant increase % cellular proliferation by 125%, 146%, and 157% in the BT-DMEM-P1, BT-DMEM-P2, and BT-DMEM-P3, respectively as compared to the control group. Overall, data suggested that Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi has shown the significant improvement of the stem cell growth and cell count in plant callus, mouse, and human stem cells. Therefore, The Trivedi Effect® can be used as a complementary and alternate therapy for the stem cells regeneration, and improvement of tissue injury that might be every effective against various autoimmune human diseases such as arthritis, osteoarthritis, and different neurodegenerative disorders.