Impact of Consciousness Energy Healing Treatment on Herbomineral Formulation in Different Skin Health Parameters

The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.

MTT cell viability assay data showed more than 70% cells were viable, which indicated that the test formulation was safe and nontoxic in all the tested concentrations. The percent cell proliferation using BrdU assay was significantly increased by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Collagen level was significantly increased by 15.90% in the BT-DMEM + UT-Test formulation at 1.25 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly increased by 9.10% in the UT-DMEM + BT-Test formulation at 5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation group at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Melanin level was significantly (p≤0.001) reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the UT-DMEM + UT-Test formulation group. Anti-wrinkling effect using UV-B induced stress in HFF-1 cells showed that the Biofield Energy Treated test formulation was increased by 21.32%, 23.81%, and 7.20% at 0.63, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Scratch assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Altogether, the Biofield Energy Treated test formulation (The Trivedi Effect®) has shown significant protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Therefore, the Consciousness Energy Healing based herbomineral formulation might be suitable for the development of herbal cosmetics, which could be useful for the management of wounds and various skin related disorders viz. syringoma, photosensitivity, urticaria, hives, warts, abscess, callus, acne, chickenpox, eczema, rosacea, seborrheic dermatitis, athlete's foot, psoriasis, erythema, contact dermatitis, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.