Evaluation of the Effects of Biofield Energy Healing Based Herbomineral Formulation on Various Biomarkers in Male Sprague Dawley Rats: Potential Role of the Trivedi Effect®

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The IgM, IgG, CD4+, and CD8+ were altered in the Biofield Energy Treated test formulation (G4) compared to the disease control (G2). TLC was significantly increased by 19.35% in the G4 compared to the G2. Neutrophil was significantly increased by 31.53%, 29.73%, 31.53%, and 33.84% in the G4, untreated test formulation (G5), G6, and G7, respectively compared to the G2. The levels of total cholesterol (TC), triglycerides (TG), and very low density lipoprotein (VLDL) were significantly lowered by 18.18% (p≤0.01), 46.33% (p≤0.01), and 46.41% (p≤0.01), respectively in the G4 compared to the G2. Additionally, the levels of TC, TG, low density lipoprotein (LDL), and VLDL were significantly reduced by 25.50% (p≤0.001), 96.39% (p≤0.001), 13.91%, and 55.08% (p≤0.001), respectively in the G6 compared to the G2. Alkaline phosphatase (ALP) was significantly reduced by 33.65% (p≤0.001) in the G4 compared to the G2. Moreover, the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), ALP, and creatine kinase myocardium band (CK-MB) were significantly decreased by 29.44% (p≤0.05), 25.54% (p≤0.01), 27.97% (p≤0.01), and 36.07% (p≤0.01), respectively in the G7 group compared to the G2. Testosterone was significantly increased by 83.68%, 305.90%, 1271.03% (p<0.001) and 745.35% (p<0.05) in the G4, G5, G6 and G7, respectively compared to the G2. The level of catalase enzyme was significantly increased by 57.91% (p<0.01) and 98.51% (p<0.001) in Biofield Energy Treatment per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7), respectively compared to the G2. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Based on the current study findings, the IgM level was significantly increased by 13.07% and 16.34% in the Biofield Energy Treated test formulation (G4) and Biofield Energy Treated test formulation at day -15 (G7), respectively compared to the disease control group (G2). The expression of the cellular biomarkers like CD4+ and CD8+ was altered minimally in the Biofield Energy Treated test formulation group compared to the disease control. The Biofield Energy Treated test formulation group (G4) showed significant increase the levels of TLC (19.35%) and neutrophils (31.53%), and reduce the levels of lipid biomarkers like TC, TG, and VLDL by 18.18%, 46.33%, and 46.41%, respectively compared to the disease control group (G2). Moreover, the levels of SGOT, SGPT, ALP, and CK-MB were significantly decreased by 29.44%, 25.54%, 27.97%, and 36.07%, respectively in the G7 group compared to the G2 group. The level of the hepatic enzyme like ALP was significantly reduced by 33.65% in the G4 group compared to the G2 group. Further, testosterone was significantly increased by 83.68% and 745.35% in the G4 and G7, respectively compared to the G2 group. The level of catalase was significantly increased by 57.91% and 98.51% in the Biofield Energy Treatment group per se at day -15 (G6) and G7, respectively compared to the G2 group.

Overall, it can be concluded that the novel herbomineral formulation and animals per se after treatment with the Trivedi Effect®-Biofield Energy Healing remotely by the seven Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties. Therefore, the Biofield Energy Treated test formulation and animals per se may act as an effective anti-inflammatory and immunomodulatory product, and it can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants, and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and Quality of Life (QoL).