Evaluation of Phosphodiesterase-5 Inhibitory Potential of Biofield Energy Treated DMEM by Determining cGMP Level in Human Endothelial Cell Line

Erectile dysfunction is a common disorder found in men, which occurs due to multiple factors such as psychogenic, hormonal imbalance, and neurovascular disturbances. The present investigation was undertaken to examine the effect of a Consciousness Energy Healing based DMEM medium on the Human Endothelial Hybrid Cell Line (EA. hy926) to evaluate the level of cyclic guanosine monophosphate (cGMP). The test item (DMEM medium) was divided into three parts, first part received a one-time Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Alice Branton and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times the Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. The level of cGMP for the inhibition of PDE-5 enzyme was assessed using cGMP ELISA assay kit (colorimetric). Sildenafil citrate, used as positive control, which showed a significant increase of cGMP levels in Ea. hy926 cells. The one-time Biofield Energy Healing Treated DMEM (BT-I) showed 29.92% alteration, while the two-times Biofield Energy Healing Treated DMEM (BT-II) showed a significantly (p≤0.001) increased level of intracellular cGMP by 307.09% in Ea. hy926 cells compared to the untreated DMEM group. Overall, experimental data suggested that the two-times Biofield Treated DMEM showed a significantly improved level of cGMP compared with the one-time Biofield Treated DMEM group. Therefore, data indicated that the Biofield Energy Healing Treatment can be used to treat erectile dysfunction along with other sexual disorders such as orgasmic disorders, female sexual arousal disorder, fetishistic disorder, frotteuristic disorder, hypoactive sexual desire disorder, sex addiction, sexual masochism and sadism, vaginismus, voyeuristic disorder, premature or delayed ejaculation.

Erectile dysfunction is a common, multifactorial disorder, which is associated with a wide number of factors such as aging, organic and psychogenic conditions such as hypertension, hypercholesterolemia, diabetes mellitus, cardiovascular disease, and depression. Modern therapy is not attractive due to its lot of life-threating side-effects. Thus, Biofield Energy Healing Therapy can be used to treat erectile dysfunction (ED). The study results showed that the two-times Biofield Energy Treated DMEM significantly (p≤0.001) increased the level of the intracellular cGMP levels by 307.09% in the Ea. hy926 cells compared to the untreated DMEM group. The Biofield Energy Treated (The Trivedi Effect®) DMEM has significant impact on cGMP level, which might be due to the significant inhibition of the PDE-5 enzyme. Therefore, the Consciousness Energy Healing Therapy might be a suitable alternative treatment option for ED. It can also be useful for the management of various sexual disorders viz. dyspareunia, exhibitionistic disorder, female and male orgasmic disorders (delay or absence of orgasm), female sexual arousal disorder (inability to become physically aroused or excited during sexual activity), fetishistic disorder, frotteuristic disorder, hypoactive sexual desire disorder, sex addiction, vaginismus, premature or delayed ejaculation (or sexual malfunction or sexual disorder) improve normal sexual activity, including physical pleasure, desire, preference, arousal or orgasm, desire disorders (lack of sexual desire or interest in sex), and neurological disorders, hormonal imbalances, sexual performance, marital or relationship problems, effects of a past sexual trauma, depression, feelings of guilt, pain disorders (pain during intercourse).