Evaluation of Biofield Energy Treated Vitamin D3 in Human Osteoblasts Cells

Bone formation, remodeling and healing processes involves a coordinated action of different cell types. Vitamin D and calcium significantly improves the patients' quality of life with respect to various bone disorders and comorbidities most prevalent in the elderly. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vicki Lynn Kindy and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. Cell viability using 3-(4, 5-diamethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium) (MTT) data showed that the test items were found to be safe. ALP level was significantly increased by 621.2%, 638.9%, and 102.2% at 1, 10, and 50 µg/mL, respectively in UT-DMEM+BT-TI, as compared to the untreated group. Collagen content was significantly increased up to 284.6%, 134%, and 183.4% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 109.6% and 159.6% increased collagen in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 120% and 229.4% at 50 and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 254.7% and 78.1% in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL as compared with the untreated group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

In conclusion, overall data suggested that Biofield Energy Healing based vitamin D3 and DMEM have significant effect on bone health in MG-63 cell line. MTT assay data showed a significant improved cell viability with more than 80% in all the test samples along with improved cell viability as compared with the untreated group. In addition, Biofield Energy Treated test samples showed a significant improved level of ALP by 621.2%, 638.9%, and 102.2% in UT-DMEM+BT-TI at 1, 10, and 50 µg/mL, respectively, while 151.9% and 202.1% at 1 and 10 µg/mL in BT-DMEM+UT-TI group. In addition, the ALP level was increased 215.4% and 127.4% at 1 and 10 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. The level of collagen was significantly increased by 284.6%, 134%, and 183.4% at 1, 10, and 50 µg/mL, respectively in UT-DMEM+BT-TI while 109.6%, 55.7%, and 40.8% increased collagen was reported at 1, 10, and 50, respectively in BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, the level of collagen was increased by 159.6%, 86.6%, and 26.1% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test group. Similarly, the bone mineralization percent was significantly increased by 120% and 229.4% at 50 and 100 µg/mL, respectively in UT-DMEM+BT-TI group, 254.7% and 78.1% in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL as compared with the untreated test group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, calcium and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-stress, anti-aging, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, cell cycling and proliferation, hormonal balance, neurotransmission, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, stress etc. with a safe therapeutic index to improve overall health and quality of life.