Effects of The Biofield Energy Healing Treated Novel Test Formulation for Skin Health and Aging

Skin reflects the correlation between the overall inner-health and aging status, which is the complex biological process influenced by the various factors. The present work evaluated the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the test formulation and cell medium (DMEM) for various skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation was consisted of seven ingredients viz. zinc chloride, ferrous sulfate, copper chloride, magnesium gluconate, pyridoxine HCl, vitamin B12, and vitamin D3 . The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by a renowned Biofield Energy Healer, Alice Branton and defined as the Biofield Treated (BT) sample, while other was denoted as the Untreated (UT) samples. MTT assay using test formulation showed greater than 76% cell viability, suggested safe and nontoxic profile at tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 127.92% and 121.20% at 50 µg/mL in the BT-DMEM + UTTest formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 13.08%, and 15.98% at 50 and 100 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group.

The level of elastin was increased by 28.09% at 50 µg/mL in the UT-DMEM + BT-Test formulation group, compared with the untreated group. Hyaluronic acid level was significantly (p≤0.001) increased by 86.10% and 35.14% at 10 and 50 µg/mL, respectively in the BT-DMEM + BT-Test formulation group as compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 16.96% at 10 µg/mL in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect exhibited improved cell viability by 65.63% and 69.95% at 1 and 10 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with the untreated group. In vitro wound healing activity using scratch assay significantly improved healing rate and cell migration upto 58.55% in the HFF-1 cells in the UT-DMEM + BT-Test formulation group, while upto 231.25% in HaCaT cells lines in UT-DMEM + BT-Test formulation group compared with the untreated group. In conclusion, The Trivedi Effect® based test formulation and DMEM have the significant capacity to improve overall skin health, reversal of aging process and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.

The experimental data of skin health study of The Trivedi Effect®-Consciousness Energy Healing based test formulation and DMEM data suggested significant increase in cell viability (using MTT assay) with 84%, 85%, and 76% in HaCaT, HFF-1, and B16F10 cells all the tested concentrations, indicating that the test formulation was found safe and nontoxic. Besides, BrdU assay showed 127.92% and 121.20% increase cellular proliferation (at 50 µg/mL) in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively while 18.46% and 21.34% increase in cellular proliferation in the BT-DMEM + UTTest formulation and BT-DMEM + BT-Test formulation groups, respectively at 100 µg/mL compared to the UT-DMEM + UTTest formulation group. The level of collagen was increased after The Trivedi Effect®-Consciousness Energy Healing Treatment by 2.41%, 13.08%, and 15.98% at 10, 50, and 100 µg/mL, respectively, in the UT-DMEM + BT-Test formulation group, while 2.94% and 5.55% increase collagen level at 50 and 100 µg/mL, respectively, in the BT-DMEM + BT-Test formulation group with respect to the UT-DMEM + UT-Test formulation group. However, the level of elastin was significantly increased by 28.90% at concentration 50 µg/mL in the UT-DMEM + BT-Test formulation compared to the UT-DMEM + UT-Test formulation group.

In addition, the level of HA in HFF-1 cells showed 20.18% at concentration 1 µg/mL in UT-DMEM + BT-Test formulation group, while 29.61%, and 12.41% at concentration 1 and 10 µg/ mL, respectively in the BT-DMEM + UT-Test formulation group compared with the untreated group. Further, the level of HA has been significantly (p≤0.001) improved in the BT-DMEM + BT-Test formulation group by 6.59%, 86.10%, and 35.14% at concentration 1, 10, and 50 µg/mL, respectively compared with the UT-DMEM + UT-Test formulation group. The melanin level was significantly reduced by 16.96% at 10 µg/mL in the UTDMEM + BT-Test formulation group, while 11.61% reduced at 10 µg/mL in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Anti-wrinkling potential with respect to UV-B induced stress showed an increase cell viability by 33.66%, 19.87%, and 18.46% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. In addition, cell viability was significantly increased by 65.63% and 69.95% at concentration 1 and 10 µg/mL, respectively, in BTDMEM + BT-Test formulation group. Wound healing scratch assay showed a significant migration of fibroblast and keratinocytes cells with increase covered area. HFF-1 cellular migration was increased up to 58.55% (100 µg/ mL) in UT-DMEM + BT-Test formulation group compared with the untreated group. However, HaCaT cells showed significant improved cell migration up to 231.25% at 50 µg/mL in BT-DMEM + UT-Test formulation group, while up to 206.25% at 50 µg/mL in BT-DMEM + BT-Test formulation group compared with the untreated group, which suggest its significant role in wound healing and other skin-related diseases such as anti-wrinkling, anti-aging, and skin whitening.

In conclusion, Biofield Energy Treated test formulation and DMEM showed significant improved skin health and helps in reversal of ageing process. Overall, The Trivedi Effect®- Consciousness Energy Healing Treatment can be used as a Complementary and Alternative Medicine (CAM) treatment with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing has significant capacity in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.