Biofield Energy Healing Based Vitamin D3: An Improved Overall Bone Health Activity in MG-63 Cell Line

The potential of The Trivedi Effect®-Consciousness Energy Healing vitamin D3 and DMEM medium was investigated in human bone osteosarcoma cells (MG-63). The Test Items (TI) i.e. vitamin D3 and DMEM were separated into two parts. Bone health parameters were tested such as Alkaline Phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Karen Byrnes Allen and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the Untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 71% with safe and nontoxic profile among test samples on MG-63 cell line. The level of ALP was significantly increased by116.4% at 50µg/mL in the UT-DMEM+BT-TI group, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respectively in the BT-DMEM+UT-TI group. In addition, ALP level was increased by 42.3% and 90.6% at 10 and 50 μg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated group. The level of collagen was significantly increased by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively in the UT-DMEM+BT-TI group, while 22.6% and 190.6% at 0.1 and 1µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 140.6% and 39.9% at 0.1 and 1 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 128.7% at 50 μg/mL, in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μg/mL, respectively as compared with the untreated group. Thus, the study results concluded that The Trivedi Effect® Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.

The experimental results of cell viability data using MTT as-say showed more than 71% cells were viable and Consciousness Energy Healing based vitamin D3 further improved the cell viabil-ity. Thus, MTT data indicated that the test samples were safe and nontoxic in all the tested concentrations. However, various bone health parameters were significantly improved such as ALP was increased by 116.4% at 50 μg/mL in the UT-DMEM+BT-TI, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respective-ly in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, BT-DMEM+BT-TI group showed an increased ALP level by 42.3% and 90.6% at 10 and 50 µg/mL, respectively. The level of collagen was significantly in-creased by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively in the UT-DMEM+BT-TI, while 22.6%, 190.6%, and 1.6% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI group. The level of collagen was increased by 140.6% and 39.9% at 0.1 and 1µg/mL, respectively in BT-DMEM+BT-TI group as compared with the un-treated test item and DMEM group. Similarly, the bone mineraliza-tion percent was significantly increased by 128.7% at 550 μg/mL in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 µg/mL, respectively as compared with the untreated group. The Bone health parameters were significantly improved among the Biofield Energy Treated vitamin D3 test samples in MG-63 cells. Overall, the Biofield Ener-gy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. colla-gen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional sup-plement, which could be useful for the management of various bone related disorders viz. osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3can be useful as anti-inflammatory, anti-aging, anti-stress, an-ti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentia-tion, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Be-sides, it can also be utilized in hormonal imbalance, aging, and var-ious immune related disease conditions such as Multiple Sclerosis, Alzheimer’s Disease, Asthma, Atherosclerosis, Pernicious Anemia, Aplastic Anemia, Diverticulitis, Graves’ Disease, Dermatomyositis, Dermatitis, stress, Irritable Bowel Syndrome, Systemic Lupus Ery-thematosus, Hepatitis, Hashimoto Thyroiditis,Diabetes, Myasthe-nia Gravis, Ulcerative Colitis, Sjogren Syndrome, Parkinson’s Dis-ease, etc. with a safe therapeutic index to improve overall health, and quality of life.