A Prospective Study on Bone Health: Effect of Biofield Energy Treated Vitamin D3 Using MG-63 Cell Line

The aim of the present study was to evaluate the significant role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Laura Nelson Streicher and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). MTT assay for cell viability showed that increased range 76% to 139% cell viability with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 232.7% (1 µg/mL), 156.4% (100 µg/mL) and 262.5% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. The level of collagen was significantly increased by 145.8% and 151.6% at 10 and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 177.1%, 235.6%, and 165.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 252.1% at 1 µg/mL as compared with the untreated test item and DMEM group.The percent of bone mineralization was significantly increased by 92.8%, 65.8%, and 32.2% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 76.8%, 132.5%, and 7.6% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 92.8%, 38.6%, and 12.3% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Biofield Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improvesbone mineralization, and calcium absorption in wide range of bone disordersalong with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Bone health parameters were significantly improved among the Biofield Energy Treated vitamin D3 test samples in MG-63 cells. Cell viability testing using MTT assay data showed significant improved cell viability with more than 90% among various experimental groups. Other bone health parameters such as the level of ALP was increased by 232.7%, 82.5%, and 34.1% at 1, 10, and 100 µg/mL, respectively in the UT-DMEM+BT-TI, while 156.4% at 100 µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, BT-DMEM+BT-TI group showed an increased ALP level by 262.5% at 100 µg/mL. The level of collagen was significantly increased by 14.6%, 145.8%, and 151.6% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 177.1%, 235.6%, and 165.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. The level ofcollagen was increased by 252.1% at 1 µg/mL in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 92.8%, 65.8%, and 32.2% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 6.8%, 132.5%, and 7.6% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 92.8%, 38.6%, and 12.3% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 is useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influences the cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Multiple Sclerosis, Aplastic Anemia, Asthma, Hepatitis, Ulcerative Colitis, Alzheimer’s Disease, Dermatomyositis, Dermatitis, Parkinson’s Disease, Irritable Bowel Syndrome, Pernicious Anemia, Sjogren Syndrome, Graves’ Disease, Diabetes, Myasthenia Gravis, and Atherosclerosis.